Araştırma Makalesi
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Serological and molecular detection of Leek yellow stripe virus infecting onion (Allium cepa L.) and leek (Allium ampeloprasum L.) in Ankara, Turkey

Yıl 2020, Cilt: 60 Sayı: 1, 49 - 56, 31.03.2020
https://doi.org/10.16955/bitkorb.622150

Öz

Onion (Allium cepa L.) has a high economic value in Turkey and it is widely used as main ingredient in Turkish menus. During 2018, 25% of 1.930.695 tons onion production in Turkey was produced in Ankara province. Recently, virus-like symptoms had been observed on onion fields but so far there was no study on onion viral diseases in Ankara province. Leek yellow stripe virus (LYSV) mainly infects leek (Allium ampeloprasum L.) but also infects and potentially causes yield reduction on onion. In August 2018, 45 onion samples show typical viral infection symptoms such as leaf stripes, leaf malformation, yellowing, and dwarfing and six leek samples showing severe yellow leaf stripe were collected from fields in Ankara province. 6 onion and 2 leek samples were positive to LYSV infection in DAS-ELISA. Total nucleic acid was then extracted from samples to be used in RT-PCR using a pair of specific primer to amplify a fragment of LYSV CP region. 6 onion and 2 leek samples were also positive in the RT-PCR by forming specific bands of 1020 bp on agarose gel. The remaining samples did not produce any band on agarose gel, thus were evaluated as negative for LYSV infection. Phylogenetic analysis showed that LYSV Ankara isolates were most similar to isolates from Serbia, Argentina and Germany. This study confirmed LYSV infection in onion and leek for the first time in Ankara province, Turkey.

Destekleyen Kurum

Bilimsel Araştırma Projeleri Koordinatörlüğü, Ankara Üniversitesi

Proje Numarası

18H0447001

Teşekkür

Authors are grateful to Scientific Research Projects Coordinator of Ankara University for the funding of this research (Project No. 18H0447001). This research was part of a Doctoral disertation at Plant Protection Department, Ankara University. The abstract was published in the 1. International Molecular Plant Protection Congress (10-13 April 2019, Adana, Turkey).

Kaynakça

  • Bos L., 1982. Viruses and virus diseases of Allium species. Acta Hortic., 127, 11-29.
  • Brunt A. A., Crabtree K., Dallwitz M. J., Gibbs A. J., Watson L., 1996. Leek yellow stripe potyvirus. Plant viruses online: Description and lists from the VIDE database. http://bio-mirror.im.ac.cn/mirrors/pvo/vide/descr437.htm (Date accessed 11.05.2019).
  • Centre for Agriculture and Bioscience International., 2001. Neotoxoptera formosana, Distribution map no. 620.
  • Elnagar S., El-Sheikh M. A., Abd El-Wahab A. S. E., 2011. Effect of Natural Infection with Onion Yellow Dwarf Virus (OYDV) on Yield of Onion and Garlic Crops in Egypt. Journal of Life Sciences 5, 634-638.
  • Fajardo T. V. M., Nishijima M., Buso J. A., Torres A. C., Ávila A. C., Resende R. O., 2001. Garlic viral complex: identification of Potyviruses and Carlavirus in central Brazil. Fitopatologia Brasileira 26, 619-626.
  • Fidan H., 2010. Sarımsak, Soğan ve Pırasadaki Virüs Hastalıklarını Saptanması ve Taşköprü 56 Sarımsak Tipinin En Yaygın Virüse Karşı Reaksiyonunun Belirlenmesi (in Turkish). Cukurova University, Institute of Natural and Applied Sciences, Doctoral disertation, 183 p., Adana, Turkey.
  • Khadair A. H., Evans I. R., Choban B., 2002. Identification of Aster yellows phytoplasma in garlic and green onion by PCR-based methods. Microbiol. Res. 157, 161-167.
  • Korkmaz S., Cevik B., 2009. Leek yellow stripe virus newly reported in Turkey. Plant Pathology 58, 787.
  • Lot H., Chovelon V., Souche S., Delecolle B., 1998. Effects of onion yellow dwarf and leek yellow stripe viruses on symptomatology and yield loss of three French garlic cultivars. Plant Dis. 82, 1381-1385.
  • Presting G. G., Smith O. P., Brown C. R., 1995. Resistance to Potato leafroll virus in potato plants transformed with the coat protein gene or with vector control constructs. Phytopathology 85, 436-442.
  • Shahraeen N., Lesemann D. E., Ghotbi T., 2008. Survey for viruses infecting onion, garlic and leek crops in Iran. Bulletin OEPP/EPPO Bulletin 38, 131–135.
  • Sevik M. A., Akcura C., 2013. Viruses occurring in onion crop in Amasya province, the major onion producing region in Turkey. Indian J. Virol. 24(1), 78-81.
  • Thangasamy A., Khade Y., Singh M., 2018. Nutrient Management in Onion and Garlic. Journal of Allium Research 1(1), 107-115.
  • Torrico A. K., Celli M. G., Conci L. R., Conci V.C., 2015. Incidence of Garlic common latent virus in Argentina, and phylogenetic and recombination analyses of isolates. Pesq. Agropec. Brasilia 50(5), 363-373.
  • Turkish Statistical Institute (TurkStat), 2019. http://www.tuik.gov.tr/Start.do (Date accessed 15.08.2019).
  • Ward L. I., Perez-Egusquiza Z., Fletcher J. D., Clover G. R. G., 2009. A survey of viral diseases of Allium crops in New Zealand. Australasian Plant Pathology 38, 533–539.

Ankara’da soğan (Allium cepa L.) ve pırasayı (Allium ampeloprasum L.) enfekte eden Pırasa sarı çizgi virüsü’nün (Leek yellow stripe potyvirus) serolojik ve moleküler olarak tanılanması

Yıl 2020, Cilt: 60 Sayı: 1, 49 - 56, 31.03.2020
https://doi.org/10.16955/bitkorb.622150

Öz

Soğan (Allium cepa L.) Türkiye’de yüksek derecede ekonomik öneme sahip olup, Türk menülerinde yaygın olarak kullanılmaktadır. 2018 yılında 1.930.695 tonluk Türkiye üretiminin %25’i Ankara’da yapılmıştır. Son yıllarda soğan tarlalarında virus benzeri belirtiler gözlenmiş ancak Ankara bölgesinde soğan virus hastalıkları üzerine bir çalışma yapılmamıştır. Leek yellow stripe virus (LYSV) esas olarak pırasayı (Allium ampeloprasum L.) enfekte etmekle birlikte soğanı da hastalandırmakta ve verim düşüklüğüne de neden olmaktadır. 2018 yılı ağustos ayında, tipik virüs belirtisi, örneğin yaprakta şeritler, yaprak deformasyonu, sararma ve cüceleşme belirtileri gösteren 45 soğan örneği ve yapraklarda şiddetli sarı çizgi belirtisi tasıyan 6 pırasa örneği tarladan toplanmıştır. 45 soğan örneğinin 6 tanesi, 6 pırasa örneğinin 2 tanesi DAS-ELISA çalışmalarında LYSV’ye karsı pozitif sonuç vermiştir. Örneklerden elde edilen toplam nükleik asit RT-PCR’da LYSV’ye karşı geliştirilen örtü proteinine spesifik primerlerle amplifiye edilmiştir. 6 soğan ve 2 pırasa örneği RT-PCR çalışmalarında da pozitif sonuç vermiş ve agarose jelde 1020 bp büyüklüğünde bir bant oluşturmuşlardır. Diğer örnekler agarose jelde bir bant oluşturmamış ve bunlar LYSV enfeksiyonu açısından negatif olarak değerlendirilmişlerdir. Yapılan filogenetik analiz sonucunda LYSV Ankara izolatlarının Sırbistan, Arjantin ve Almanya izolatları ile benzerlik gösterdiği görülmüştür. Bu araştırma ile LYSV enfeksiyonu Ankara ilinde ilk olarak kanıtlanmış bulunmaktadır.

Proje Numarası

18H0447001

Kaynakça

  • Bos L., 1982. Viruses and virus diseases of Allium species. Acta Hortic., 127, 11-29.
  • Brunt A. A., Crabtree K., Dallwitz M. J., Gibbs A. J., Watson L., 1996. Leek yellow stripe potyvirus. Plant viruses online: Description and lists from the VIDE database. http://bio-mirror.im.ac.cn/mirrors/pvo/vide/descr437.htm (Date accessed 11.05.2019).
  • Centre for Agriculture and Bioscience International., 2001. Neotoxoptera formosana, Distribution map no. 620.
  • Elnagar S., El-Sheikh M. A., Abd El-Wahab A. S. E., 2011. Effect of Natural Infection with Onion Yellow Dwarf Virus (OYDV) on Yield of Onion and Garlic Crops in Egypt. Journal of Life Sciences 5, 634-638.
  • Fajardo T. V. M., Nishijima M., Buso J. A., Torres A. C., Ávila A. C., Resende R. O., 2001. Garlic viral complex: identification of Potyviruses and Carlavirus in central Brazil. Fitopatologia Brasileira 26, 619-626.
  • Fidan H., 2010. Sarımsak, Soğan ve Pırasadaki Virüs Hastalıklarını Saptanması ve Taşköprü 56 Sarımsak Tipinin En Yaygın Virüse Karşı Reaksiyonunun Belirlenmesi (in Turkish). Cukurova University, Institute of Natural and Applied Sciences, Doctoral disertation, 183 p., Adana, Turkey.
  • Khadair A. H., Evans I. R., Choban B., 2002. Identification of Aster yellows phytoplasma in garlic and green onion by PCR-based methods. Microbiol. Res. 157, 161-167.
  • Korkmaz S., Cevik B., 2009. Leek yellow stripe virus newly reported in Turkey. Plant Pathology 58, 787.
  • Lot H., Chovelon V., Souche S., Delecolle B., 1998. Effects of onion yellow dwarf and leek yellow stripe viruses on symptomatology and yield loss of three French garlic cultivars. Plant Dis. 82, 1381-1385.
  • Presting G. G., Smith O. P., Brown C. R., 1995. Resistance to Potato leafroll virus in potato plants transformed with the coat protein gene or with vector control constructs. Phytopathology 85, 436-442.
  • Shahraeen N., Lesemann D. E., Ghotbi T., 2008. Survey for viruses infecting onion, garlic and leek crops in Iran. Bulletin OEPP/EPPO Bulletin 38, 131–135.
  • Sevik M. A., Akcura C., 2013. Viruses occurring in onion crop in Amasya province, the major onion producing region in Turkey. Indian J. Virol. 24(1), 78-81.
  • Thangasamy A., Khade Y., Singh M., 2018. Nutrient Management in Onion and Garlic. Journal of Allium Research 1(1), 107-115.
  • Torrico A. K., Celli M. G., Conci L. R., Conci V.C., 2015. Incidence of Garlic common latent virus in Argentina, and phylogenetic and recombination analyses of isolates. Pesq. Agropec. Brasilia 50(5), 363-373.
  • Turkish Statistical Institute (TurkStat), 2019. http://www.tuik.gov.tr/Start.do (Date accessed 15.08.2019).
  • Ward L. I., Perez-Egusquiza Z., Fletcher J. D., Clover G. R. G., 2009. A survey of viral diseases of Allium crops in New Zealand. Australasian Plant Pathology 38, 533–539.
Toplam 16 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Makaleler
Yazarlar

Adyatma Santosa

Filiz Ertunç

Proje Numarası 18H0447001
Yayımlanma Tarihi 31 Mart 2020
Gönderilme Tarihi 19 Eylül 2019
Kabul Tarihi 16 Aralık 2019
Yayımlandığı Sayı Yıl 2020 Cilt: 60 Sayı: 1

Kaynak Göster

APA Santosa, A., & Ertunç, F. (2020). Serological and molecular detection of Leek yellow stripe virus infecting onion (Allium cepa L.) and leek (Allium ampeloprasum L.) in Ankara, Turkey. Plant Protection Bulletin, 60(1), 49-56. https://doi.org/10.16955/bitkorb.622150
AMA Santosa A, Ertunç F. Serological and molecular detection of Leek yellow stripe virus infecting onion (Allium cepa L.) and leek (Allium ampeloprasum L.) in Ankara, Turkey. Plant Protection Bulletin. Mart 2020;60(1):49-56. doi:10.16955/bitkorb.622150
Chicago Santosa, Adyatma, ve Filiz Ertunç. “Serological and Molecular Detection of Leek Yellow Stripe Virus Infecting Onion (Allium Cepa L.) and Leek (Allium Ampeloprasum L.) in Ankara, Turkey”. Plant Protection Bulletin 60, sy. 1 (Mart 2020): 49-56. https://doi.org/10.16955/bitkorb.622150.
EndNote Santosa A, Ertunç F (01 Mart 2020) Serological and molecular detection of Leek yellow stripe virus infecting onion (Allium cepa L.) and leek (Allium ampeloprasum L.) in Ankara, Turkey. Plant Protection Bulletin 60 1 49–56.
IEEE A. Santosa ve F. Ertunç, “Serological and molecular detection of Leek yellow stripe virus infecting onion (Allium cepa L.) and leek (Allium ampeloprasum L.) in Ankara, Turkey”, Plant Protection Bulletin, c. 60, sy. 1, ss. 49–56, 2020, doi: 10.16955/bitkorb.622150.
ISNAD Santosa, Adyatma - Ertunç, Filiz. “Serological and Molecular Detection of Leek Yellow Stripe Virus Infecting Onion (Allium Cepa L.) and Leek (Allium Ampeloprasum L.) in Ankara, Turkey”. Plant Protection Bulletin 60/1 (Mart 2020), 49-56. https://doi.org/10.16955/bitkorb.622150.
JAMA Santosa A, Ertunç F. Serological and molecular detection of Leek yellow stripe virus infecting onion (Allium cepa L.) and leek (Allium ampeloprasum L.) in Ankara, Turkey. Plant Protection Bulletin. 2020;60:49–56.
MLA Santosa, Adyatma ve Filiz Ertunç. “Serological and Molecular Detection of Leek Yellow Stripe Virus Infecting Onion (Allium Cepa L.) and Leek (Allium Ampeloprasum L.) in Ankara, Turkey”. Plant Protection Bulletin, c. 60, sy. 1, 2020, ss. 49-56, doi:10.16955/bitkorb.622150.
Vancouver Santosa A, Ertunç F. Serological and molecular detection of Leek yellow stripe virus infecting onion (Allium cepa L.) and leek (Allium ampeloprasum L.) in Ankara, Turkey. Plant Protection Bulletin. 2020;60(1):49-56.

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