Öz
Aim: The continuing outbreak of coronavirus disease (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) poses enormous challenges for health care workers. Health care workers are a high-risk population for transmission of SARS-CoV-2 infection from patients or other healthcare workers. In this research, it was aimed to determine the rate of IgG antibody formation against SARS-CoV-2 by taking into account the gender, age group, occupational group, unit of work, reported symptoms and family contact history of healthcare workers in Düzce.
Material and Methods: The study group was randomly formed from healthcare professionals working actively in the pandemic units in Düzce. A questionnaire questioning sociodemographic information was applied to the participants and blood samples were taken.Antibody levels were studied with the microELISA method using the SARS-CoV-2 IgG (Euroimmun, Germany) kit in microbiology laboratory.
Results: SARS-CoV-2 IgG positivity was found in 25 (27%) of the 93 healthcare workers included in the study. 18 (72%) of those who were positive for SARS CoV-2 IgG antibody had passed COVID-19 asymptomatically. 17 (68%) of those who were positive for SARS CoV-2 IgG antibody did not have a family history of contact with a person positive for COVID-19.While the median value of antibody levels was 2.90 in symptomatic patients, this value was found as 1.31 in asymptomatic patients.
Conclusion: As a result, it was observed that the majority of those who were included in the study and had antibody positivity had the infection asymptomatically and the antibody levels in these individuals were lower than those who were symptomatic.
Anahtar Kelimeler
Kaynakça
- Plebani M, Padoan A, Fedeli U, Schievano E, Vecchiato E, Lippi G, et al. SARS-CoV-2 serosurvey in health care workers of the Veneto Region. Clin Chem Lab Med. 2020; 26: 58(12): 2107-11. https://doi.org/10.1515/cclm-2020-1236.
- Basteiro ALG, Moncunill G, Tortajada M, Tortajada M, Vidal M, Guinovart C, et al. Seroprevalence of antibodies against SARS-CoV-2 among health care workers in a large Spanish reference hospital. Nat Commun. 2020; 11(1): 3500. https://doi.org/ 10.1038/s41467-020-17318-x.3.
- Brischetto A, Robson J. Testing for COVID-19. Aust Prescr. 2020; 43(6): 204-8.
- Alpdagtas S, Ilhan E, Uysal E, Sengor M, Ustundag CB, Gunduz O. Evaluation of current diagnostic methods for COVID-19. APL Bioeng. 2020; 4(4): 041506. https://doi.org /10.1063/5.0021554.
- Augustine R, Das S, Hasan A, Abhilash S, Abdul Salam S, Augustine P, et al. Rapid antibody-based COVID-19 mass surveillance: Relevance, challenges, and prospects in a pandemic and post-pandemic world. J Clin Med. 2020; 9(10): 3372.
- Peeling RW, Wedderburn CJ, Garcia PJ, Boeras D, Fongwen N, Nkengasong J, et al. Serology testing in the COVID-19 pandemic response. Lancet Infect Dis. 2020; 20: e245-49. https://doi.org/10.1016/S1473-3099(20)30517-X.
- Venugopal U, Jilani N, Rabah S, Shariff MA, Jawed M, Batres AM, et al. SARS-CoV-2 seroprevalence among health care workers in a New York City hospital: A cross-sectional analysis during the COVID-19 pandemic. Int J Infect Dis. 2020; 102: 63-9. https://doi.org/10.1016/j.ijid.2020.10.036.
- Korth J, Wilde B, Dolff S, Anastasiou OE, Krawczyk A, Jahn M, et al. SARS-CoV-2-specific antibody detection in healthcare workers in Germany with direct contact to COVID-19 patients. Journal of Clinical Virology. 2020; 128: 104437. https://doi.org /10.1016/j.jcv.2020.104437.
- Sotgiu G, Barassi A, Miozzo M, Saderi L, Piana A, Orfeo N, et al. SARS-CoV-2 specific serological pattern in healthcare workers of an Italian COVID-19 forefront hospital. BMC Pulmonary Medicine. 2020; 20(1): 1-6.
- Weis S, Scherag A, Baier M, Kiehntopf M, Kamradt T, Kolanos S, et al. Antibody response using six different serological assays in a completely PCR-tested community after a COVID-19 outbreak - The CoNAN study. Clin Microbiol Infect. 2020; S 1198-743 X (20) 30705-9. https://doi.org 10.1016/j.cmi.2020.11.009.
- Kılınç Ç, Çöplü N, Gözalan A, Sönmez C, Çalışır B, Gül M, Aygün Ahlatçıoğlu Z. TMC 2020 Çevrimiçi Mikrobiyoloji Sempozyumu; 2020. 130-1.
Öz
Amaç: Şiddetli akut solunum sendromu koronavirüs 2'nin (SARS-CoV-2) neden olduğu ve devam eden eden koronavirüs hastalığı (COVID-19) salgını, sağlık çalışanları için büyük zorluklar oluşturmaktadır. Sağlık çalışanları, hastalardan veya diğer sağlık çalışanlarından SARS-CoV-2 enfeksiyonu bulaşı açısından yüksek riskli bir popülasyondur. Bu çalışmada, Düzce ilindeki sağlık çalışanlarının cinsiyet, yaş grubu, meslek grubu, çalıştığı birim, bildirdiği semptomlar ve aile içi temas öyküsü durumu göz önüne alınarak SARS-CoV-2'ye karşı IgG yapısındaki antikor oluşturma oranlarının belirlenmesi amaçlandı.
Gereç ve Yöntemler: Çalışma grubu, Düzce ilindeki pandemi birimlerinde aktif olarak görev yapan sağlık çalışanlarından randomize olarak oluşturuldu. Katılımcılara sosyodemografik bilgilerin sorgulandığı bir anket uygulandı ve kan örnekleri alındı. Antikor düzeyleri SARS-CoV-2 IgG (Euroimmun, Almanya) kiti kullanılarak mikro ELİSA yöntemi ile mikrobiyoloji laboratuvarında çalışıldı.
Bulgular: Çalışmaya dahil edilen 93 sağlık personelinden 25’inde (%27) SARS-CoV-2 IgG pozitifliği saptandı. SARS CoV-2 IgG antikoru pozitif olanların 18’i (%72) asemptomatik olarak COVID-19’u geçirmişti. SARS CoV-2 IgG antikoru pozitif olanların 17’sinde ise (%68) COVID-19 pozitif kişi ile aile içi temas öyküsü yoktu. Semptomatik geçirenlerde antikor düzeylerinin median değeri 2,90 olarak saptanmışken, asemptomatik geçirenlerde bu değer 1,31 olarak bulundu.
Sonuç: Sonuç olarak çalışmaya dahil edilen ve antikor pozitifliği olan sağlık çalışanlarının büyük çoğunluğunun asemptomatik olarak enfeksiyonu geçirdiği ve bu kişilerdeki antikor düzeylerinin semptomatik olanlardan daha düşük olduğu görülmüştür.
Anahtar Kelimeler
Kaynakça
- Plebani M, Padoan A, Fedeli U, Schievano E, Vecchiato E, Lippi G, et al. SARS-CoV-2 serosurvey in health care workers of the Veneto Region. Clin Chem Lab Med. 2020; 26: 58(12): 2107-11. https://doi.org/10.1515/cclm-2020-1236.
- Basteiro ALG, Moncunill G, Tortajada M, Tortajada M, Vidal M, Guinovart C, et al. Seroprevalence of antibodies against SARS-CoV-2 among health care workers in a large Spanish reference hospital. Nat Commun. 2020; 11(1): 3500. https://doi.org/ 10.1038/s41467-020-17318-x.3.
- Brischetto A, Robson J. Testing for COVID-19. Aust Prescr. 2020; 43(6): 204-8.
- Alpdagtas S, Ilhan E, Uysal E, Sengor M, Ustundag CB, Gunduz O. Evaluation of current diagnostic methods for COVID-19. APL Bioeng. 2020; 4(4): 041506. https://doi.org /10.1063/5.0021554.
- Augustine R, Das S, Hasan A, Abhilash S, Abdul Salam S, Augustine P, et al. Rapid antibody-based COVID-19 mass surveillance: Relevance, challenges, and prospects in a pandemic and post-pandemic world. J Clin Med. 2020; 9(10): 3372.
- Peeling RW, Wedderburn CJ, Garcia PJ, Boeras D, Fongwen N, Nkengasong J, et al. Serology testing in the COVID-19 pandemic response. Lancet Infect Dis. 2020; 20: e245-49. https://doi.org/10.1016/S1473-3099(20)30517-X.
- Venugopal U, Jilani N, Rabah S, Shariff MA, Jawed M, Batres AM, et al. SARS-CoV-2 seroprevalence among health care workers in a New York City hospital: A cross-sectional analysis during the COVID-19 pandemic. Int J Infect Dis. 2020; 102: 63-9. https://doi.org/10.1016/j.ijid.2020.10.036.
- Korth J, Wilde B, Dolff S, Anastasiou OE, Krawczyk A, Jahn M, et al. SARS-CoV-2-specific antibody detection in healthcare workers in Germany with direct contact to COVID-19 patients. Journal of Clinical Virology. 2020; 128: 104437. https://doi.org /10.1016/j.jcv.2020.104437.
- Sotgiu G, Barassi A, Miozzo M, Saderi L, Piana A, Orfeo N, et al. SARS-CoV-2 specific serological pattern in healthcare workers of an Italian COVID-19 forefront hospital. BMC Pulmonary Medicine. 2020; 20(1): 1-6.
- Weis S, Scherag A, Baier M, Kiehntopf M, Kamradt T, Kolanos S, et al. Antibody response using six different serological assays in a completely PCR-tested community after a COVID-19 outbreak - The CoNAN study. Clin Microbiol Infect. 2020; S 1198-743 X (20) 30705-9. https://doi.org 10.1016/j.cmi.2020.11.009.
- Kılınç Ç, Çöplü N, Gözalan A, Sönmez C, Çalışır B, Gül M, Aygün Ahlatçıoğlu Z. TMC 2020 Çevrimiçi Mikrobiyoloji Sempozyumu; 2020. 130-1.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Sağlık Kurumları Yönetimi |
| Bölüm | Araştırma Makaleleri |
| Yazarlar | |
| Yayımlanma Tarihi | 20 Eylül 2021 |
| Gönderilme Tarihi | 29 Aralık 2020 |
| Yayımlandığı Sayı | Yıl 2021 Cilt: 11 Sayı: 3 |
