Araştırma Makalesi
BibTex RIS Kaynak Göster

Evaluation of Different DNA İsolation Techniques in Honey, Pollen And Propolis Samples Belonging to Çankırı Province

Yıl 2023, Cilt: 13 Sayı: 1, 582 - 591, 01.03.2023
https://doi.org/10.21597/jist.1201801

Öz

Honey is a natural and complex nutrient produced by collecting and processing secretions obtained from insects that feed on nectar or plant extract by bees. Honey, which is one of the most consumed natural foods, has many benefits in terms of human health. One of the most effective ways to access this information is to isolate the DNA found in honey by analyzing some of the specific gene sequences it contains. However, due to the inhibitors it contains, which are quite viscous in nature, there are a number of problems and limitations in the analysis of DNA in honey. In present study, it was aimed to compare 3 different isolation techniques, CTAB, TRIzol methods and DNeasy® Plant Pro Kit in honey, pollen and propolis samples and to recommend the most appropriate method for these samples. As a result of the spectrophotometric method and gel analyzes to image the genomic DNA, DNA isolation from propolis samples could not be performed with CTAB and DNeasy® Plant Pro Kit methods, and DNA isolation from honey sample could not be performed in TRIzol method. The TRIzol method was successful compared to the other two methods for the propolis sample. Especially for the propolis sample with a very dense chemical structure, the TRIzol method was studied for the first time and yield was obtained. The data obtained as a result of this study support the implementation of such applications for other types of honey and honey-based products produced in our country and thus the raising of the quality standards of honey, which has an important place in the economy of our country.

Kaynakça

  • Asghar, U., Malik, M. F., Anwar, F., Javed, A. ve Raza, A. (2015). DNA Extraction from Insects by Using Different Techniques: A Review. Advances in Entomology, 3(4), 132-138.
  • Bankova, V., Popova, M. ve Trusheva, B. (2016). New emerging fields of application of propolis. Macedonian Journal of Chemistry and Chemical Engineering, 35(1), 1-11.
  • Blaxter, M. W. ve Davey, J. L. (2010). RADseq: Next-generation population genetics. Briefings in Functional Genomics, 9(5-6), 416-423.
  • Bruni, I., Galimberti, A., Caridi, L., Scaccabarozzi, D., De Mattia, F., Casiraghi, M. ve Labra, M. (2015). A DNA barcoding approach to identify plant species in multiflower honey. Food Chemistry, 170(2015), 308-315.
  • Chomczynski, P. (1993). A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. BioTechniques, 15(3), 532-537.
  • de Vere, N., Jones, L. E., Gilmore, T., Moscrop, J., Lowe, A., Smith, D., Hegarty, M. J., Creer, S. ve Ford, C. R. (2017). Using DNA metabarcoding to investigate honeybee foraging reveals limited flower use despite high floral availability. Scientific Reports,7(1), 1-10.
  • Ferreiro-González, M., Espada-Bellido, E., Guillén-Cueto, L., Palma, M., Barroso, C. G. ve Barbero, G. F. (2018). Rapid quantification of honey adulteration by visible-near infrared spectroscopy combined with chemometrics. Talanta, 188(2018), 288-92.
  • Furlong, M. J. (2015). Knowing your enemies: Integrating molecular and ecological methods to assess the impact of arthropod predators on crop pests. Insect Science, 22(1), 6-19.
  • Gomes, S., Dias, L. G., Moreira, L. L., Rodrigues, P. Ve Estevinho, L. (2010). Physicochemical, microbiological and antimicrobial properties of commercial honeys from Portugal. Food and Chemical Toxicology, 48(2), 544-548.
  • Guertler, P., Eicheldinger, A., Muschler, P., Goerlich, O. ve Busch, U. (2013). Automated DNA extraction from pollen in honey. Food Chemistry, 149(2014), 302-306.
  • Kress, W. J., García-Robledo, C., Uriarte, M. ve Erickson, D. L. (2015). DNA barcodes for ecology, evolution, and conservation. Trends in Ecology and Evolution, 30(1), 25-35.
  • Luo, C., Zou, X., Li, Y., Sun, C., Jiang, Y. ve Wu, Z. (2011). Determination of flavonoids in propolis-rich functional foods by reversed phase high performance liquid chromatography with diode array detection. Food Chemistry, 127(1), 314-320.
  • Mardis, E. R. (2017). DNA sequencing technologies: 2006–2016. Nature Protocols, 12(1), 213-218.
  • Meo, S. A., Al-Asiri, S. A., Mahesar, A. L. ve Ansari, M. J. (2017). Role of honey in modern medicine. Saudi Journal of Biological Sciences, 24(5), 975-978.
  • Muradian, L. B. D. A., Barth, O. M., Dietemann, V., Eyer, M., Freitas, A. D. S. D., Martel, A. C., Marcazzan, G. L., Marchese, C. M., Caretta, C. M., Mate, A. P., ve Reybroeck, W. (2020). Standard methods for Apis mellifera honey research. Journal of Apicultural Research, 59(3), 1-62.
  • Olivieri, C., Morata, I., Rollo, F. ve Luciani, S. (2012). Tracking Plant, Fungal, and Bacterial DNA in Honey Specimens. Journal of Forensic Science, 57(1), 222-227.
  • Oryan, A., Alemzadeh, E. ve Moshiri, A. (2018). Potential role of propolis in wound healing: Biological properties and therapeutic activities. Biomedicine&Pharmacotherapy, 98(2018), 469-483.
  • Saxena, S., Gautam, S. ve Sharma, A. (2010). Physical, biochemical and antioxidant properties of some Indian honeys. Food Chemistry, 118(2), 391-397.
  • Silici, S. (2019). Honeybee products and apitherapy. Turkish Journal of Agriculture-Food Science and Technology, 7(9), 1249-1262.
  • Soares, S., Amaral, J. S., Oliveira, M. B. P. P., ve Mafra, I. (2014). Improving DNA isolation from honey for the botanical origin identification. Food Control, 48(2015), 130-136.
  • Stanimirova, I., Üstün, B., Cajka, T., Riddelova, R., Hajslova, J., Buydens, L. M. C. ve Walczak, B. (2010). Tracing the geographical origin of honeys based on volatile compounds profiles assessment using pattern recognition techniques. Food Chemistry, 118(1), 171-176.
  • Van Dijk, E. L., Auger, H., Jaszczyszyn, Y. ve Thermes, C. (2014). Ten years of next-generation sequencing technology. Trends in Genetics, 30(9), 418-426.
  • Waiblinger, H. U., Ohmenhaeuser, M., Meissner, S., Schillinger, M., Pietsch, K., Goerlich, O., Mankertz, J., Lieske, K., ve Broll, H. (2012). In-house and interlaboratory validation of a method for the extraction of DNA from pollen in honey. Journal für Verbraucherschutz und Lebensmittelsicherheit, 7(3), 243-254.
  • Waykar, B. ve Alqadhi, Y. A. (2016). Beekeping and bee products; Boon for humen health and wealth. Indian Journal of Pharmaceutical and Biological Research, 4(3), 20-27.
  • Wingfield, M. J., ve Klein, H. (2012). DNA extraction techniques for DNA barcoding of minute gall-inhabiting wasps. Molecular Ecology Resources, 12(2012), 109-115.
Yıl 2023, Cilt: 13 Sayı: 1, 582 - 591, 01.03.2023
https://doi.org/10.21597/jist.1201801

Öz

Kaynakça

  • Asghar, U., Malik, M. F., Anwar, F., Javed, A. ve Raza, A. (2015). DNA Extraction from Insects by Using Different Techniques: A Review. Advances in Entomology, 3(4), 132-138.
  • Bankova, V., Popova, M. ve Trusheva, B. (2016). New emerging fields of application of propolis. Macedonian Journal of Chemistry and Chemical Engineering, 35(1), 1-11.
  • Blaxter, M. W. ve Davey, J. L. (2010). RADseq: Next-generation population genetics. Briefings in Functional Genomics, 9(5-6), 416-423.
  • Bruni, I., Galimberti, A., Caridi, L., Scaccabarozzi, D., De Mattia, F., Casiraghi, M. ve Labra, M. (2015). A DNA barcoding approach to identify plant species in multiflower honey. Food Chemistry, 170(2015), 308-315.
  • Chomczynski, P. (1993). A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. BioTechniques, 15(3), 532-537.
  • de Vere, N., Jones, L. E., Gilmore, T., Moscrop, J., Lowe, A., Smith, D., Hegarty, M. J., Creer, S. ve Ford, C. R. (2017). Using DNA metabarcoding to investigate honeybee foraging reveals limited flower use despite high floral availability. Scientific Reports,7(1), 1-10.
  • Ferreiro-González, M., Espada-Bellido, E., Guillén-Cueto, L., Palma, M., Barroso, C. G. ve Barbero, G. F. (2018). Rapid quantification of honey adulteration by visible-near infrared spectroscopy combined with chemometrics. Talanta, 188(2018), 288-92.
  • Furlong, M. J. (2015). Knowing your enemies: Integrating molecular and ecological methods to assess the impact of arthropod predators on crop pests. Insect Science, 22(1), 6-19.
  • Gomes, S., Dias, L. G., Moreira, L. L., Rodrigues, P. Ve Estevinho, L. (2010). Physicochemical, microbiological and antimicrobial properties of commercial honeys from Portugal. Food and Chemical Toxicology, 48(2), 544-548.
  • Guertler, P., Eicheldinger, A., Muschler, P., Goerlich, O. ve Busch, U. (2013). Automated DNA extraction from pollen in honey. Food Chemistry, 149(2014), 302-306.
  • Kress, W. J., García-Robledo, C., Uriarte, M. ve Erickson, D. L. (2015). DNA barcodes for ecology, evolution, and conservation. Trends in Ecology and Evolution, 30(1), 25-35.
  • Luo, C., Zou, X., Li, Y., Sun, C., Jiang, Y. ve Wu, Z. (2011). Determination of flavonoids in propolis-rich functional foods by reversed phase high performance liquid chromatography with diode array detection. Food Chemistry, 127(1), 314-320.
  • Mardis, E. R. (2017). DNA sequencing technologies: 2006–2016. Nature Protocols, 12(1), 213-218.
  • Meo, S. A., Al-Asiri, S. A., Mahesar, A. L. ve Ansari, M. J. (2017). Role of honey in modern medicine. Saudi Journal of Biological Sciences, 24(5), 975-978.
  • Muradian, L. B. D. A., Barth, O. M., Dietemann, V., Eyer, M., Freitas, A. D. S. D., Martel, A. C., Marcazzan, G. L., Marchese, C. M., Caretta, C. M., Mate, A. P., ve Reybroeck, W. (2020). Standard methods for Apis mellifera honey research. Journal of Apicultural Research, 59(3), 1-62.
  • Olivieri, C., Morata, I., Rollo, F. ve Luciani, S. (2012). Tracking Plant, Fungal, and Bacterial DNA in Honey Specimens. Journal of Forensic Science, 57(1), 222-227.
  • Oryan, A., Alemzadeh, E. ve Moshiri, A. (2018). Potential role of propolis in wound healing: Biological properties and therapeutic activities. Biomedicine&Pharmacotherapy, 98(2018), 469-483.
  • Saxena, S., Gautam, S. ve Sharma, A. (2010). Physical, biochemical and antioxidant properties of some Indian honeys. Food Chemistry, 118(2), 391-397.
  • Silici, S. (2019). Honeybee products and apitherapy. Turkish Journal of Agriculture-Food Science and Technology, 7(9), 1249-1262.
  • Soares, S., Amaral, J. S., Oliveira, M. B. P. P., ve Mafra, I. (2014). Improving DNA isolation from honey for the botanical origin identification. Food Control, 48(2015), 130-136.
  • Stanimirova, I., Üstün, B., Cajka, T., Riddelova, R., Hajslova, J., Buydens, L. M. C. ve Walczak, B. (2010). Tracing the geographical origin of honeys based on volatile compounds profiles assessment using pattern recognition techniques. Food Chemistry, 118(1), 171-176.
  • Van Dijk, E. L., Auger, H., Jaszczyszyn, Y. ve Thermes, C. (2014). Ten years of next-generation sequencing technology. Trends in Genetics, 30(9), 418-426.
  • Waiblinger, H. U., Ohmenhaeuser, M., Meissner, S., Schillinger, M., Pietsch, K., Goerlich, O., Mankertz, J., Lieske, K., ve Broll, H. (2012). In-house and interlaboratory validation of a method for the extraction of DNA from pollen in honey. Journal für Verbraucherschutz und Lebensmittelsicherheit, 7(3), 243-254.
  • Waykar, B. ve Alqadhi, Y. A. (2016). Beekeping and bee products; Boon for humen health and wealth. Indian Journal of Pharmaceutical and Biological Research, 4(3), 20-27.
  • Wingfield, M. J., ve Klein, H. (2012). DNA extraction techniques for DNA barcoding of minute gall-inhabiting wasps. Molecular Ecology Resources, 12(2012), 109-115.
Toplam 25 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Ziraat, Veterinerlik ve Gıda Bilimleri
Bölüm Moleküler Biyoloji ve Genetik / Moleculer Biology and Genetic
Yazarlar

Ebru Derelli Tüfekçi 0000-0003-1097-8574

Erken Görünüm Tarihi 24 Şubat 2023
Yayımlanma Tarihi 1 Mart 2023
Gönderilme Tarihi 9 Kasım 2022
Kabul Tarihi 23 Aralık 2022
Yayımlandığı Sayı Yıl 2023 Cilt: 13 Sayı: 1

Kaynak Göster

APA Derelli Tüfekçi, E. (2023). Evaluation of Different DNA İsolation Techniques in Honey, Pollen And Propolis Samples Belonging to Çankırı Province. Journal of the Institute of Science and Technology, 13(1), 582-591. https://doi.org/10.21597/jist.1201801
AMA Derelli Tüfekçi E. Evaluation of Different DNA İsolation Techniques in Honey, Pollen And Propolis Samples Belonging to Çankırı Province. Iğdır Üniv. Fen Bil Enst. Der. Mart 2023;13(1):582-591. doi:10.21597/jist.1201801
Chicago Derelli Tüfekçi, Ebru. “Evaluation of Different DNA İsolation Techniques in Honey, Pollen And Propolis Samples Belonging to Çankırı Province”. Journal of the Institute of Science and Technology 13, sy. 1 (Mart 2023): 582-91. https://doi.org/10.21597/jist.1201801.
EndNote Derelli Tüfekçi E (01 Mart 2023) Evaluation of Different DNA İsolation Techniques in Honey, Pollen And Propolis Samples Belonging to Çankırı Province. Journal of the Institute of Science and Technology 13 1 582–591.
IEEE E. Derelli Tüfekçi, “Evaluation of Different DNA İsolation Techniques in Honey, Pollen And Propolis Samples Belonging to Çankırı Province”, Iğdır Üniv. Fen Bil Enst. Der., c. 13, sy. 1, ss. 582–591, 2023, doi: 10.21597/jist.1201801.
ISNAD Derelli Tüfekçi, Ebru. “Evaluation of Different DNA İsolation Techniques in Honey, Pollen And Propolis Samples Belonging to Çankırı Province”. Journal of the Institute of Science and Technology 13/1 (Mart 2023), 582-591. https://doi.org/10.21597/jist.1201801.
JAMA Derelli Tüfekçi E. Evaluation of Different DNA İsolation Techniques in Honey, Pollen And Propolis Samples Belonging to Çankırı Province. Iğdır Üniv. Fen Bil Enst. Der. 2023;13:582–591.
MLA Derelli Tüfekçi, Ebru. “Evaluation of Different DNA İsolation Techniques in Honey, Pollen And Propolis Samples Belonging to Çankırı Province”. Journal of the Institute of Science and Technology, c. 13, sy. 1, 2023, ss. 582-91, doi:10.21597/jist.1201801.
Vancouver Derelli Tüfekçi E. Evaluation of Different DNA İsolation Techniques in Honey, Pollen And Propolis Samples Belonging to Çankırı Province. Iğdır Üniv. Fen Bil Enst. Der. 2023;13(1):582-91.