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The Electrospinning of Different Hemostatic Agents and Their Effectiveness

Yıl 2024, Cilt: 5 Sayı: 1, 1 - 9, 16.04.2024

Başak Ünver Koluman Mahmed Sari Njjar Ahmet Koluman

Öz

The purpose of this study is to present effective solutions for severe bleeding and tissue damage occurring during situations such as conflicts, disasters, and crises. The aim is to produce nanofibers that can serve as a hemostatic wound dressing, capable of both accelerating wound healing and controlling bleeding. Nanofibers are produced through the electrospinning method by combining hemostatic agents such as adrenaline, Transamin® (TXA), and Ankaferd Blood Stopper® (ABS) with polyvinyl alcohol (PVA) polymer. The morphology, chemical bonding, and hemostatic activity of these nanofibers are extensively analyzed. The feasibility of these nanofibers for medical applications, particularly as wound dressings, was investigated. Results: Field emission scanning electron microscopy (FESEM) images reveal that increased concentrations of adrenaline, TXA, and ABS result in the formation of beaded fibers. While ABS/PVA and TXA/PVA nanofibers have similar average diameters, ABS/PVA exhibits a more beaded morphology. According to hemostatic activity tests, clotting times were similar for adrenaline/PVA and TXA/PVA nanofibers, whereas ABS/PVA nanofibers exhibited a shorter clotting time. Among the findings, adrenaline/PVA nanofibers had the longest clotting time at 4.088 seconds. On the other hand, ABS/PVA nanofibers had the shortest clotting time at 3.819 seconds. An effective hemostatic agent should be able to stop bleeding within 2 minutes after application to the wound site in in vitro settings, without requiring mixing or preparation, and should be easily applicable to wounded areas. The developed hemostatic nanofibers demonstrated the ability to form clots within seconds. The resulting nanofibers from this study will not only contribute to public health but also significantly enhance survival processes.

Anahtar Kelimeler

Astringents Hemorrhage Bleeding Time Ankaferd Blood Stopper Adrenaline Tranexamic Acid

Kaynakça

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Farklı Hemostatik Ajanların Elektroeğirme Yöntemiyle Üretimi ve Etkinliği

Yıl 2024, Cilt: 5 Sayı: 1, 1 - 9, 16.04.2024

Başak Ünver Koluman Mahmed Sari Njjar Ahmet Koluman

Öz

Bu çalışmanın amacı, çatışmalar, felaketler ve krizler gibi durumlar sırasında meydana gelen ciddi kanama ve doku hasarına yönelik etkili çözümler sunmak amacıyla hem yara iyileşmesini hızlandırabilen hem de kanamayı kontrol edebilen bir hemostatik yara örtüsü olarak kullanılabilecek nanolifler üretmektir. Nanolifler, elektroeğirme yöntemiyle adrenalin, Transamin® (TXA) ve Ankaferd Kan Durdurucu® (ABS) gibi hemostatik maddeler ile polivinil alkol (PVA) polimerinin birleşimiyle elde edilmektedir. Çalışmada nanoliflerin morfolojisi, kimyasal bağ yapısı ve hemostatik aktivitesi ayrıntılı şekilde analiz edilmektedir. Bu nanoliflerin tıbbi uygulamalarda, özellikle yara örtüsü olarak kullanılabilirliği araştırılmıştır. Alan emisyonlu taramalı elektron mikroskobu (FESEM) görüntülerine göre, adrenalin, TXA ve ABS konsantrasyonunun artmasıyla boncuklu lifler oluşmaya başlamıştır. ABS/PVA ve TXA/PVA nanoliflerinin ortalama çapları aynı olmasına rağmen, ABS/PVA daha fazla boncuklu morfolojiye sahiptir. Hemostatik aktivite testlerine göre, adrenalin/PVA ve TXA/PVA nanoliflerinin pıhtılaşma süreleri benzerdi; ancak ABS/PVA nanoliflerinin pıhtılaşma süresi daha kısaydı. Bulgulara göre, adrenalin/PVA nanolifleri en uzun pıhtılaşma süresine sahipti ve bu süre 4.088 saniyeydi. Öte yandan, ABS/PVA nanolifleri en kısa pıhtılaşma süresine sahipti ve bu süre 3.819 saniyeydi. İyi bir hemostat, in vitro uygulamalarda yara bölgesine uygulandıktan sonra 2 dakika içinde kanamayı durdurabilmeli, karıştırma veya hazırlama gerektirmemeli ve yaralı bölgelere basitçe uygulanabilmelidir. Bu çalışmada elde edilen hemostatik nanoliflerin saniyeler içinde pıhtı oluşturabilme yeteneği gösterilmiştir. Geliştirilen nanolifler, sadece halk sağlığı açısından değil, aynı zamanda hayatta kalma süreçlerine güçlü bir katkı sağlayacaktır.

Anahtar Kelimeler

Kanamayı Durdurucular Kanama Zamanı Ankaferd Kan Durdurucu Adrenalin Traneksamik Asit

Kaynakça

  • [1] Çolak, S., Altan, A., Akbulut, N. (2018). Lokal Hemostatik Ajanlar. Uluslararası Diş Hekimliği Bilimleri Dergisi, (3), 147-152.
  • [2] Sierra, C., Moreno, M. U., & García‐Ruiz, J. C. (2022). The physiology of hemostasis. Blood Coagulation & Fibrinolysis, 33(S1), S1–S2. https://doi.org/10.1097/mbc.0000000000001099
  • [3] Sruthi, S.M., (2021, August 4). How do hemostatics work? - uses, side effects, drug names. RxList. https://www.rxlist.com/how_do_hemostatics_work/drug-class.htm
  • [4] Stannard, A., Morrison, J. J., Scott, D., Ivatury, R. A., Ross, J. D., & Rasmussen, T. E. (2013). The epidemiology of noncompressible torso hemorrhage in the wars in Iraq and Afghanistan. The Journal of Trauma and Acute Care Surgery, 74(3), 830–834. https://doi.org/10.1097/ta.0b013e31827a3704
  • [5] Eastridge, B. J., Hardin, M. O., Cantrell, J. A., Oetjen-Gerdes, L., Zubko, T., Mallak, C. T., Wade, C. E., Simmons, J. W., Mace, J. E., Mabry, R., Bolenbaucher, R., & Blackbourne, L. H. (2011). Died of Wounds on the Battlefield: Causation and implications for Improving Combat Casualty care. Journal of Trauma-injury Infection and Critical Care, 71(1), S4–S8. https://doi.org/10.1097/ta.0b013e318221147b
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  • [7] Martin, M. J., Oh, J. S., Currier, H., Tai, N., Beekley, A. C., Eckert, M. J., & Holcomb, J. B. (2009). An analysis of In-Hospital deaths at a modern combat support Hospital. Journal of Trauma-injury Infection and Critical Care, 66(4), S51–S61. https://doi.org/10.1097/ta.0b013e31819d86ad
  • [8] Bakhsheshi‐Rad, H. R., Ismail, A. F., Aziz, M., Hadisi, Z., Omidi, M., & Chen, D. (2019). Antibacterial activity and corrosion resistance of Ta2O5 thin film and electrospun PCL/MgO-Ag nanofiber coatings on biodegradable Mg alloy implants. Ceramics International, 45(9), 11883–11892. https://doi.org/10.1016/j.ceramint.2019.03.071
  • [9] Balusamy, B., Senthamizhan, A. & Uyar, T. (2020). Electrospun Nanofibers for Wound Dressing and Tissue Engineering Applications. Hacettepe Journal of Biology and Chemistry, The 100 Year of Polymers, 459-481. DOI: 10.15671/hjbc.789186
  • [10] Zhao, Y., Qiu, Y., Wang, H., Chen, Y., Jin, S., & Chen, S. (2016). Preparation of nanofibers with renewable polymers and their application in wound dressing. International Journal of Polymer Science, 2016.
  • [11] Archana, D., Singh, B. K., Dutta, J., & Dutta, P. K. (2015). Chitosan-PVP-nano silver oxide wound dressing: in vitro and in vivo evaluation. International journal of biological macromolecules, 73, 49-57.
  • [12] Lu, Z., Gao, J., He, Q., Wu, J., Liang, D., Yang, H., & Chen, R. (2017). Enhanced antibacterial and wound healing activities of microporous chitosan-Ag/ZnO composite dressing. Carbohydrate polymers, 156, 460-469.
  • [13] Fatahian, R., Mirjalili, M., Khajavi, R., Rahimi, M. K., & Nasirizadeh, N. (2020). Fabrication of antibacterial and hemostatic electrospun PVA nanofibers for wound healing. SN Applied Sciences, 2, 1-7.
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Toplam 56 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Biyomedikal Mühendisliği (Diğer), Polimer Bilimi ve Teknolojileri
Bölüm Araştırma Makaleleri
Yazarlar

Başak Ünver Koluman 0000-0003-1106-5021

Mahmed Sari Njjar 0000-0003-2494-1086

Ahmet Koluman 0000-0001-5308-8884

Yayımlanma Tarihi 16 Nisan 2024
Yayımlandığı Sayı Yıl 2024 Cilt: 5 Sayı: 1

Kaynak Göster

APA Ünver Koluman, B., Njjar, M. S., & Koluman, A. (2024). The Electrospinning of Different Hemostatic Agents and Their Effectiveness. Research Journal of Biomedical and Biotechnology, 5(1), 1-9.
AMA Ünver Koluman B, Njjar MS, Koluman A. The Electrospinning of Different Hemostatic Agents and Their Effectiveness. RJBB. Nisan 2024;5(1):1-9.
Chicago Ünver Koluman, Başak, Mahmed Sari Njjar, ve Ahmet Koluman. “The Electrospinning of Different Hemostatic Agents and Their Effectiveness”. Research Journal of Biomedical and Biotechnology 5, sy. 1 (Nisan 2024): 1-9.
EndNote Ünver Koluman B, Njjar MS, Koluman A (01 Nisan 2024) The Electrospinning of Different Hemostatic Agents and Their Effectiveness. Research Journal of Biomedical and Biotechnology 5 1 1–9.
IEEE B. Ünver Koluman, M. S. Njjar, ve A. Koluman, “The Electrospinning of Different Hemostatic Agents and Their Effectiveness”, RJBB, c. 5, sy. 1, ss. 1–9, 2024.
ISNAD Ünver Koluman, Başak vd. “The Electrospinning of Different Hemostatic Agents and Their Effectiveness”. Research Journal of Biomedical and Biotechnology 5/1 (Nisan 2024), 1-9.
JAMA Ünver Koluman B, Njjar MS, Koluman A. The Electrospinning of Different Hemostatic Agents and Their Effectiveness. RJBB. 2024;5:1–9.
MLA Ünver Koluman, Başak vd. “The Electrospinning of Different Hemostatic Agents and Their Effectiveness”. Research Journal of Biomedical and Biotechnology, c. 5, sy. 1, 2024, ss. 1-9.
Vancouver Ünver Koluman B, Njjar MS, Koluman A. The Electrospinning of Different Hemostatic Agents and Their Effectiveness. RJBB. 2024;5(1):1-9.
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